Logo

We have a project available for an enthusiastic MSc student!

We study the role of monocytes and macrophages in atherosclerosis, ranging from cardiovascular clinical trials to fundamental research on the epigenetic control of macrophages. The currently available fundamental research project aims to investigate how the Polycomb Repressive Complex 2, which acts on the repressive H3K27Me3 modification, regulates macrophage activation.


Objectives:

I) Generation of CRISPR-Cas9 knock-out cell lines

II) Functional assessment of the effect of the knock-outs on macrophage characteristics.


You will generate knock-out cell lines by cloning, sequencing, genome editing, transduction, transfection, cell culture, and flow cytometry-assisted cell sorting. Once the knock-out cell lines are obtained, various techniques will be at your disposal to functionally assess the knock-out effect on macrophage characteristics, including but not limited to RT-qPCR, ELISA, flow cytometry, and Western blot.


As part of our group, you will join departmental seminars, journal clubs, weekly work meetings, group discussions, and regular social events.

If you would like to acquire molecular biology skills during a 5+ months internship or want more information, please contact r.w.m.kempkes@amsterdamumc.nl or a.e.neele@amsterdamumc.nl


Share this message

Latest news Medical Biochemistry Amsterdam

By duda-wsm February 11, 2025
UvA Synergy Program Awarded to Vascular Immunology Research Consortium An interdisciplinary team of researchers from the Faculty of Medicine (FdG) and the Faculty of Science (FNWI) has been awarded a synergy grant from UvA to advance groundbreaking research in vascular immunology. The multidisciplinary team aims to bridge the gap between fundamental science and clinical application, translating basic scientific discoveries into clinical practice and refining clinical insights into laboratory research. The Synergy Program This synergy program led by ACS researcher Professor Jaap van Buul represents a unique opportunity to bridge the gap between fundamental research and clinical practice. Key objectives of the program include using vascular dysfunction as a predictor for inflammatory-related disease risk, identifying molecular and cellular targets, and developing tailored therapies to restore vascular immunology. The grant The grant funds six PhD positions and a senior postdoc position shared between the faculties of Sciences and Medicine. The program also aims to foster interdisciplinary master's and PhD training programs to equip the next generation of scientists with expertise spanning both faculties. Interested to know more? The program will launch with an inaugural symposium this Fall titled " You are as healthy as your vessels ". 
February 4, 2025
The Huveneers lab is recruiting a PhD student
Show More
Share by: